Koleratoksiini ja munuainen

Pharmacol Toxicol. 1999 Sep;85(3):105-10. Glomerular effects of cholera toxin in isolated perfused rat kidney: a potential role for platelet activating factor.

Monteiro HS, Lima AA, Fonteles MC.

Source

Department of Physiology and Pharmacology and Clinical Research Unit-HUWC/UFC-UECE, Faculty of Medicine, Federal University of Ceará, Brazil.

Abstract

Cholera toxin (MW 84 kDa) is now considered a pharmacological tool to study the adenylyl cyclase system and a stimulus to generate platelet activating factor in the intestinal tract. We used this toxin to evaluate the renal haemodynamics, glomerular filtration function, tubular sodium transport and toxicity in isolated perfused rat kidney. Kidneys from adult male Wistar rats were isolated for perfusion. The perfusion fluid was modified Krebs-Henseleit solution and the samples were analyzed for sodium, potassium, inulin and osmolality. Clearance techniques were used to calculate physiological parameters. Cholera toxin (1.0 microg/ml) caused a significant time-dependent reduction of glomerular filtration rate and urinary flow. This toxin also caused a small, but consistent reduction in fractional proximal sodium reabsortion (toxin = 67.43+/-2.42% versus control = 79.26+/-5.80%; P<0 .025=".025" 100="100" 2086="2086" 90="90" a="a" abolished="abolished" activating="activating" addition="addition" also="also" amp="0.26+/-0.01" and="and" antagonist="antagonist" are="are" at="at" b-cyclic="b-cyclic" blocked="blocked" but="but" by="by" caused="caused" cholera="cholera" completely="completely" contrast="contrast" control="0.145+/-0.02" db-cyclic="db-cyclic" decrease="decrease" dibutyryl-cyclic="dibutyryl-cyclic" effect="effect" effects="effects" factor.="factor." factor="factor" filtration="filtration" flow.="flow." flow="flow" fractional="fractional" functional="functional" g="g" glomerular="glomerular" had="had" in="in" increase="increase" increased="increased" induced="induced" injected="injected" integrity="integrity" isolated="isolated" it="it" kidney="kidney" kidneys.="kidneys." kidneys="kidneys" m="m" maximal="maximal" mediated="mediated" microg="microg" min.="min." min="min" ml="ml" more="more" n="8," no="no" of="of" on="on" only="only" or="or" p="p" parameters="parameters" perfusate="perfusate" perfused="perfused" perfusion="perfusion" platelet="platelet" potassium="potassium" previously="previously" primarily="primarily" proximal="proximal" rat="rat" rate="rate" reabsortion="reabsortion" receptor="receptor" reduction="reduction" remained="remained" renal="renal" resistance="resistance" results="results" severe="severe" significant="significant" significantly="significantly" similar="similar" sodium="sodium" stable="stable" suggest="suggest" suggesting="suggesting" that="that" the="the" this="this" throughout="throughout" time.="time." tissue="tissue" to="to" toxin.="toxin." toxin="toxin" treated="treated" urinary="urinary" vascular="vascular" versus="versus" was="was" web="web" when="when" with="with" x="x">