750 vastausta
20 tuoreinta
Watanabe K, Suzuki H, Nishida T, Mishima M, Tachibana M, Fujishima M, Shimizu T, Watarai M.
FEMS Microbiol Ecol. 2018 Nov 1;94(11). doi: 10.1093/femsec/fiy162.
2.
Wells M, Lasek-Nesselquist E, Schoonmaker-Bopp D, Baker D, Thompson L, Wroblewski D, Nazarian E, Lapierre P, Musser KA.
Infect Genet Evol. 2018 Jul 31;65:200-209. doi: 10.1016/j.meegid.2018.07.040. [Epub ahead of print]
3.
Fleres G, Couto N, Lokate M, van der Sluis LWM, Ginevra C, Jarraud S, Deurenberg RH, Rossen JW, García-Cobos S, Friedrich AW.
Microorganisms. 2018 Jul 18;6(3). pii: E71. doi: 10.3390/microorganisms6030071.
4.
Kulkarni P, Olson ND, Paulson JN, Pop M, Maddox C, Claye E, Rosenberg Goldstein RE, Sharma M, Gibbs SG, Mongodin EF, Sapkota AR.
Sci Total Environ. 2018 Oct 15;639:1126-1137. doi: 10.1016/j.scitotenv.2018.05.178. Epub 2018 May 26.
5.
Kenzaka T, Yasui M, Baba T, Nasu M, Tani K.
Biocontrol Sci. 2018;23(2):53-59. doi: 10.4265/bio.23.53.
6.
Nikoh N, Tsuchida T, Maeda T, Yamaguchi K, Shigenobu S, Koga R, Fukatsu T.
MBio. 2018 Jun 12;9(3). pii: e00890-18. doi: 10.1128/mBio.00890-18.
7.
Mylius
M, Dreesman J, Pulz M, Pallasch G, Beyrer K, Claußen K, Allerberger F,
Fruth A, Lang C, Prager R, Flieger A, Schlager S, Kalhöfer D, Mertens E.
Int J Med Microbiol. 2018 Jul;308(5):539-544. doi: 10.1016/j.ijmm.2018.05.005. Epub 2018 May 29.
8.
- Legionella pneumophila omaa useita "muonanhankintaan" erikoistuneita kuljettajaproteiineja
Best A, Jones S, Abu Kwaik Y.
Sci Rep. 2018 May 29;8(1):8352. doi: 10.1038/s41598-018-26782-x.
9.
- Legionelloosiongelmat pysyvät uutisisia. Instituutioissa ja isoissa rakennuksissa tällaisten vesijärjestelmissä pesivien infektioiden estäminen vaatii omat ohjelmansa
Herwaldt LA, Marra AR.
Curr Opin Infect Dis. 2018 Aug;31(4):325-333. doi: 10.1097/QCO.0000000000000468.
Legionellosis remains an important public health threat. To prevent these infections, staff of municipalities and large buildings must implement effective water system management programs that reduce Legionella growth and transmission and all Medicare-certified healthcare facilities must have water management policies. In addition, we need better methods for detecting Legionella in water systems and in clinical specimens to improve prevention strategies and clinical diagnosis.
Legionellosis remains an important public health threat. To prevent these infections, staff of municipalities and large buildings must implement effective water system management programs that reduce Legionella growth and transmission and all Medicare-certified healthcare facilities must have water management policies. In addition, we need better methods for detecting Legionella in water systems and in clinical specimens to improve prevention strategies and clinical diagnosis.
10.
- Legionella pneumophila omaa ihmismitokondrian poriinin kaltaisen, jännitteestä riippuvan joniselektiivisen proteiinin, Lpg1974. Se voi muodostaa lipidikaksoiskalvoon jonia selektiivisesti läpäisevän aukon.
Younas F, Soltanmohammadi N, Knapp O, Benz R.
Biochim Biophys Acta Biomembr. 2018 Aug;1860(8):1544-1553. doi: 10.1016/j.bbamem.2018.05.008. Epub 2018 May 19.Abstract
.... Genome analyses have shown the presence of genes coding for eukaryotic like proteins in several Legionella
species. The presence of these proteins may assist L. pneumophila in
its adaptation to the eukaryotic host. We studied the characteristics of
a protein (Lpg1974) of L. pneumophila that shows remarkable homologies
in length of the primary sequence and for the identity/homology of many
amino acids to the voltage dependent anion channel (human VDAC1, Porin
31HL) of human mitochondria. Two different forms of Lpg1974 were
overexpressed in Escherichia coli and purified to homogeneity: the one
containing a putative N-terminal signal sequence and one without it.
Reconstituted protein containing the signal sequence formed
ion-permeable pores in lipid bilayer membranes with a conductance of
approximately 5.4 nS in 1 M KCl. When the predicted N-terminal signal
peptide of Lpg1974 comprising an α-helical structure similar to that at
the N-terminus of hVDAC1 was removed, the channels formed in
reconstitution experiments had a conductance of 7.6 nS in 1 M KCl. Both
Lpg1974 proteins formed pores that were voltage-dependent and
anion-selective similar to the pores formed by hVDAC1. These results
suggest that Lpg1974 of L. pneumophila is indeed a structural and
functional homologue to hVDAC1.
11.
- Neljä bakteerisukua jolla on T4SS
Esna Ashari Z, Dasgupta N, Brayton KA, Broschat SL.
PLoS One. 2018 May 9;13(5):e0197041. doi: 10.1371/journal.pone.0197041. eCollection 2018.
A thorough literature search was performed to find features that have been proposed. Feature values were calculated for datasets of known effectors and non-effectors for T4SS-containing pathogens for four genera with a sufficient number of known effectors, Legionella pneumophila, Coxiella burnetii, Brucella spp, and Bartonella spp. The features were ranked, and less important features were filtered out.
A thorough literature search was performed to find features that have been proposed. Feature values were calculated for datasets of known effectors and non-effectors for T4SS-containing pathogens for four genera with a sufficient number of known effectors, Legionella pneumophila, Coxiella burnetii, Brucella spp, and Bartonella spp. The features were ranked, and less important features were filtered out.
12.
- Ensimmäinen metagenominen tutkimnus Karachin juomavesisysteemistä.
Saleem F, Mustafa A, Kori JA, Hussain MS, Kamran Azim M.
Microb Ecol. 2018 Apr 24. doi: 10.1007/s00248-018-1192-2. [Epub ahead of print]The present metagenomic analysis of DWSS of Karachi has allowed the
evaluation of bacterial communities in source water and the water being
supplied to the city. Moreover, measurement of heavy metals in water
samples from Karachi revealed arsenic concentration according to WHO
standards which is in contrast of recent study which reported extensive
arsenic contamination in aquifers in the Indus valley plain. To the best
of our knowledge, this is the first metagenomic study of DWSS of
Karachi.
13.
- Legionella pneumophila eristyksiä 2000-2012 Kanadasta.
Fortuna A, Ramnarine R, Li A, Fittipaldi N, Frantz C, Mallo GV.
Genome Announc. 2018 Apr 12;6(15). pii: e00295-18. doi: 10.1128/genomeA.00295-18.
15.
- Eritrean kuumien lähteiden patogeeneissa Legionellaa
Ghilamicael AM, Boga HI, Anami SE, Mehari T, Budambula NLM.
PLoS One. 2018 Mar 22;13(3):e0194554. doi: 10.1371/journal.pone.0194554. eCollection 2018.
Human pathogens can survive and grow in hot springs. For water quality
assessment, Escherichia coli or Enterococci are the main thermotolerant
enteric bacteria commonly used to estimate the load of pathogenic
bacteria in water. However, most of the environmental bacteria are
unculturable thus culture methods may cause bias in detection of most
pathogens. Illumina sequencing can provide a more comprehensive and
accurate insight into environmental bacterial pathogens, which can be
used to develop better risk assessment methods and promote public health
awareness. In this study, high-throughput Illumina sequencing was used
to identify bacterial pathogens from five hot springs; Maiwooi, Akwar,
Garbanabra, Elegedi and Gelti, in Eritrea. Water samples were collected
from the five hot springs. Total community DNA was extracted from
samples using the phenol-chloroform method. The 16S rRNA gene variable
region (V4-V7) of the extracted DNA was amplified and library
construction done according to Illumina sequencing protocol. The
sequence reads (length >200 bp) from Illumina sequencing libraries
ranged from 22,091 sequences in the wet sediment sample from Garbanabra
to 155,789 sequences in the mat sample from Elegedi. Taxonomy was
assigned to each OTU using BLASTn against a curated database derived
from GreenGenes, RDPII, SILVA SSU Reference 119 and NCBI. The proportion
of potential pathogens from the water samples was highest in Maiwooi
(17.8%), followed by Gelti (16.7%), Akwar (13.6%) and Garbanabra
(10.9%). Although the numbers of DNA sequence reads from Illumina
sequencing were very high for the Elegedi (104,328), corresponding
proportion of potential pathogens very low (3.6%). Most of the potential
pathogenic bacterial sequences identified were from Proteobacteria and
Firmicutes. Legionella
and Clostridium were the most common detected genera with different
species. Most of the potential pathogens were detected from the water
samples. However, sequences belonging to Clostridium were observed more
abundantly from the mat samples. This study employed high-throughput
sequencing technologies to determine the presence of pathogenic bacteria
in the five hot springs in Eritrea.
16.
David S, Mentasti M, Parkhill J, Chalker VJ.
Clin Microbiol Infect. 2018 Sep;24(9):1020.e1-1020.e4. doi: 10.1016/j.cmi.2018.03.004. Epub 2018 Mar 13.
17.
- Legionellan effektoreissa on LotA, deubikikiinaasi.
Kubori T, Kitao T, Ando H, Nagai H.Cell Microbiol. 2018 Jul;20(7):e12840. doi: 10.1111/cmi.12840. Epub 2018 Apr 6.... Many effector proteins are expected to be involved in biogenesis and regulation of the Legionella-containing vacuole (LCV) that is highly decorated with ubiquitin. Here, we identified a Legionella deubiquitinase, designated LotA, by carrying out a genome
analysis to find proteins resembling the eukaryotic ovarian tumour
superfamily of cysteine proteases. LotA exhibits a dual ability to
cleave ubiquitin chains that is dependent on 2 distinctive catalytic
cysteine residues in the eukaryotic ovarian tumour domains. One cysteine
dominantly contributes to the removal of ubiquitin from the LCVs by its
polyubiquitin cleavage activity. The other specifically cleaves
conjugated Lys6-linked ubiquitin. After delivered by the Type 4
secretion system, LotA localises on the LCVs via its PI(3)P-binding
domain. The lipid-binding ability of LotA is crucial for ubiquitin
removal from the vacuoles. We further analysed the functional
interaction of the protein with the recently reported noncanonical
ubiquitin ligases of L. pneumophila, revealing that the effector
proteins are involved in coordinated regulation that contributes to
bacterial growth in the host cells.
18.
- Legionella sainthelensi aiheuttaa vaikeaa pneumoniaa. Genomi selvitetty Uudessa Seelannissa.
Complete Genome Sequence of Legionella sainthelensi Isolated from a Patient with Legionnaires' Disease.Slow S, Anderson T, Biggs P, Kennedy M, Murdoch D, Cree S.
Genome Announc. 2018 Feb 1;6(5). pii: e01588-17. doi: 10.1128/genomeA.01588-17.Legionella sainthelensi
is an aquatic environmental bacterium that in humans can cause
Legionnaires' disease (LD), an often severe form of pneumonia. Here, we
report the first complete genome of a L. sainthelensi clinical isolate obtained in 2001 from a patient with LD in Canterbury, New Zealand.
Currently, there are two draft genome sequences for the ATCC 35248 type
strain, which are the environmental isolates obtained near the Mt. St.
Helens volcano (GenBank accession numbers NZ_JHXP00000000 and NZ_LNYV00000000).
19.
- Neljä L. pneumophila alalajia: pneumophila, fraseri, pasculleri , raphaeli.
Kozak-Muiznieks NA, Morrison SS, Mercante JW, Ishaq MK, Johnson T, Caravas J, Lucas CE, Brown E, Raphael BH, Winchell JM.
Infect Genet Evol. 2018 Apr;59:172-185. doi: 10.1016/j.meegid.2018.02.008. Epub 2018 Feb 7.
20.
- Legionellojen fylogeneettisestä puusta
Zhan XY, Zhu QY.
PLoS One. 2018 Feb 1;13(2):e0190986. doi: 10.1371/journal.pone.0190986. eCollection 2018.
FYLOGENEETTINENPUU Phylogenetic tree.
SEKVENSSITYYPEISTÄ (ST) Sequence Typing,, standard Sequence-Based Typing .
FYLOGENEETTISTÄ ANALYYSIÄ, Phylogenetic analysis.
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